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serum free basal mammary epithelial medium  (PromoCell)
94
PromoCell serum free basal mammary epithelial medium
Representative confocal images of fluorescent PS nanoplastic internalization after the indicated time in: ( A ) primary bone marrow CD34+ sorted hematopoietic immature cells or ( B ) <t>epithelial</t> or fibroblast cells isolated from primary mammary gland obtained from healthy donors or ( C ) MCF10A-derived models representing non-transformed mammary stem cells (MCF10A-CT), early (MC26) and more transformed (M1B26) cells as previously described . Nuclei are stained in blue, actin filaments in red, PS in green. ( D ) Confocal images of PS and PET nanoplastic internalization in MCF10A after 24 h compared to non-exposed controls. Nuclei are stained blue actin filaments red, PS green, PET orange. ( E ) Schematic diagram of the nanoplastic (NPLs) long-term exposure approach and analysis of their impact. ( F ) Quantification of the sphere-forming ability of untreated (CT) MCF10A cells or following 20 weeks of exposure to PS or PET nanoplastics (NPLs), n=5. ( G ) Quantification of total stem cell-derived progenitors by E-CFC assay , data represent the total number of colonies per 250 seeded cells, n=5. ( F , G , ) Each individual datum is represented. One way ANOVA is indicated on the graph by the P values. ( H ) Representation of the mean proportion of myoepithelial, mixed and luminal colonies quantified by E-CFC assay (n = 5). Data are expressed as the percentage of each subtype with a total number of scored colonies representing 100%. ( I ) Confocal images of acini. Nuclei are stained in blue, actin filaments in red, white arrows point to acini with a disorganized structure. Measurement of acini ( J ) solidity or ( K ) Ferret diameter ratio was quantified by image analysis using Fiji imaging software, n=109 to 131 individual acini. ( J , K ) Each individual data is represented. Mann-Whitney test is indicated on the graph by the P values.
Serum Free Basal Mammary Epithelial Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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esf 921 insect cell culture medium protein free  (Expression Systems Inc)
96
Expression Systems Inc esf 921 insect cell culture medium protein free
Representative confocal images of fluorescent PS nanoplastic internalization after the indicated time in: ( A ) primary bone marrow CD34+ sorted hematopoietic immature cells or ( B ) <t>epithelial</t> or fibroblast cells isolated from primary mammary gland obtained from healthy donors or ( C ) MCF10A-derived models representing non-transformed mammary stem cells (MCF10A-CT), early (MC26) and more transformed (M1B26) cells as previously described . Nuclei are stained in blue, actin filaments in red, PS in green. ( D ) Confocal images of PS and PET nanoplastic internalization in MCF10A after 24 h compared to non-exposed controls. Nuclei are stained blue actin filaments red, PS green, PET orange. ( E ) Schematic diagram of the nanoplastic (NPLs) long-term exposure approach and analysis of their impact. ( F ) Quantification of the sphere-forming ability of untreated (CT) MCF10A cells or following 20 weeks of exposure to PS or PET nanoplastics (NPLs), n=5. ( G ) Quantification of total stem cell-derived progenitors by E-CFC assay , data represent the total number of colonies per 250 seeded cells, n=5. ( F , G , ) Each individual datum is represented. One way ANOVA is indicated on the graph by the P values. ( H ) Representation of the mean proportion of myoepithelial, mixed and luminal colonies quantified by E-CFC assay (n = 5). Data are expressed as the percentage of each subtype with a total number of scored colonies representing 100%. ( I ) Confocal images of acini. Nuclei are stained in blue, actin filaments in red, white arrows point to acini with a disorganized structure. Measurement of acini ( J ) solidity or ( K ) Ferret diameter ratio was quantified by image analysis using Fiji imaging software, n=109 to 131 individual acini. ( J , K ) Each individual data is represented. Mann-Whitney test is indicated on the graph by the P values.
Esf 921 Insect Cell Culture Medium Protein Free, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/esf 921 insect cell culture medium protein free/product/Expression Systems Inc
Average 96 stars, based on 1 article reviews
esf 921 insect cell culture medium protein free - by Bioz Stars, 2026-02
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esf 921 insect cell culture medium  (Expression Systems Inc)
96
Expression Systems Inc esf 921 insect cell culture medium
Representative confocal images of fluorescent PS nanoplastic internalization after the indicated time in: ( A ) primary bone marrow CD34+ sorted hematopoietic immature cells or ( B ) <t>epithelial</t> or fibroblast cells isolated from primary mammary gland obtained from healthy donors or ( C ) MCF10A-derived models representing non-transformed mammary stem cells (MCF10A-CT), early (MC26) and more transformed (M1B26) cells as previously described . Nuclei are stained in blue, actin filaments in red, PS in green. ( D ) Confocal images of PS and PET nanoplastic internalization in MCF10A after 24 h compared to non-exposed controls. Nuclei are stained blue actin filaments red, PS green, PET orange. ( E ) Schematic diagram of the nanoplastic (NPLs) long-term exposure approach and analysis of their impact. ( F ) Quantification of the sphere-forming ability of untreated (CT) MCF10A cells or following 20 weeks of exposure to PS or PET nanoplastics (NPLs), n=5. ( G ) Quantification of total stem cell-derived progenitors by E-CFC assay , data represent the total number of colonies per 250 seeded cells, n=5. ( F , G , ) Each individual datum is represented. One way ANOVA is indicated on the graph by the P values. ( H ) Representation of the mean proportion of myoepithelial, mixed and luminal colonies quantified by E-CFC assay (n = 5). Data are expressed as the percentage of each subtype with a total number of scored colonies representing 100%. ( I ) Confocal images of acini. Nuclei are stained in blue, actin filaments in red, white arrows point to acini with a disorganized structure. Measurement of acini ( J ) solidity or ( K ) Ferret diameter ratio was quantified by image analysis using Fiji imaging software, n=109 to 131 individual acini. ( J , K ) Each individual data is represented. Mann-Whitney test is indicated on the graph by the P values.
Esf 921 Insect Cell Culture Medium, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/esf 921 insect cell culture medium/product/Expression Systems Inc
Average 96 stars, based on 1 article reviews
esf 921 insect cell culture medium - by Bioz Stars, 2026-02
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cells edta  (Thermo Fisher)
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Thermo Fisher cells edta
Representative confocal images of fluorescent PS nanoplastic internalization after the indicated time in: ( A ) primary bone marrow CD34+ sorted hematopoietic immature cells or ( B ) <t>epithelial</t> or fibroblast cells isolated from primary mammary gland obtained from healthy donors or ( C ) MCF10A-derived models representing non-transformed mammary stem cells (MCF10A-CT), early (MC26) and more transformed (M1B26) cells as previously described . Nuclei are stained in blue, actin filaments in red, PS in green. ( D ) Confocal images of PS and PET nanoplastic internalization in MCF10A after 24 h compared to non-exposed controls. Nuclei are stained blue actin filaments red, PS green, PET orange. ( E ) Schematic diagram of the nanoplastic (NPLs) long-term exposure approach and analysis of their impact. ( F ) Quantification of the sphere-forming ability of untreated (CT) MCF10A cells or following 20 weeks of exposure to PS or PET nanoplastics (NPLs), n=5. ( G ) Quantification of total stem cell-derived progenitors by E-CFC assay , data represent the total number of colonies per 250 seeded cells, n=5. ( F , G , ) Each individual datum is represented. One way ANOVA is indicated on the graph by the P values. ( H ) Representation of the mean proportion of myoepithelial, mixed and luminal colonies quantified by E-CFC assay (n = 5). Data are expressed as the percentage of each subtype with a total number of scored colonies representing 100%. ( I ) Confocal images of acini. Nuclei are stained in blue, actin filaments in red, white arrows point to acini with a disorganized structure. Measurement of acini ( J ) solidity or ( K ) Ferret diameter ratio was quantified by image analysis using Fiji imaging software, n=109 to 131 individual acini. ( J , K ) Each individual data is represented. Mann-Whitney test is indicated on the graph by the P values.
Cells Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cells edta - by Bioz Stars, 2026-02
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proprietary cervical epithelial medium  (Innoprot Inc)
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Innoprot Inc proprietary cervical epithelial medium
Representative confocal images of fluorescent PS nanoplastic internalization after the indicated time in: ( A ) primary bone marrow CD34+ sorted hematopoietic immature cells or ( B ) <t>epithelial</t> or fibroblast cells isolated from primary mammary gland obtained from healthy donors or ( C ) MCF10A-derived models representing non-transformed mammary stem cells (MCF10A-CT), early (MC26) and more transformed (M1B26) cells as previously described . Nuclei are stained in blue, actin filaments in red, PS in green. ( D ) Confocal images of PS and PET nanoplastic internalization in MCF10A after 24 h compared to non-exposed controls. Nuclei are stained blue actin filaments red, PS green, PET orange. ( E ) Schematic diagram of the nanoplastic (NPLs) long-term exposure approach and analysis of their impact. ( F ) Quantification of the sphere-forming ability of untreated (CT) MCF10A cells or following 20 weeks of exposure to PS or PET nanoplastics (NPLs), n=5. ( G ) Quantification of total stem cell-derived progenitors by E-CFC assay , data represent the total number of colonies per 250 seeded cells, n=5. ( F , G , ) Each individual datum is represented. One way ANOVA is indicated on the graph by the P values. ( H ) Representation of the mean proportion of myoepithelial, mixed and luminal colonies quantified by E-CFC assay (n = 5). Data are expressed as the percentage of each subtype with a total number of scored colonies representing 100%. ( I ) Confocal images of acini. Nuclei are stained in blue, actin filaments in red, white arrows point to acini with a disorganized structure. Measurement of acini ( J ) solidity or ( K ) Ferret diameter ratio was quantified by image analysis using Fiji imaging software, n=109 to 131 individual acini. ( J , K ) Each individual data is represented. Mann-Whitney test is indicated on the graph by the P values.
Proprietary Cervical Epithelial Medium, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proprietary cervical epithelial medium/product/Innoprot Inc
Average 93 stars, based on 1 article reviews
proprietary cervical epithelial medium - by Bioz Stars, 2026-02
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serumfree medium esf sfm  (Expression Systems Inc)
94
Expression Systems Inc serumfree medium esf sfm
Representative confocal images of fluorescent PS nanoplastic internalization after the indicated time in: ( A ) primary bone marrow CD34+ sorted hematopoietic immature cells or ( B ) <t>epithelial</t> or fibroblast cells isolated from primary mammary gland obtained from healthy donors or ( C ) MCF10A-derived models representing non-transformed mammary stem cells (MCF10A-CT), early (MC26) and more transformed (M1B26) cells as previously described . Nuclei are stained in blue, actin filaments in red, PS in green. ( D ) Confocal images of PS and PET nanoplastic internalization in MCF10A after 24 h compared to non-exposed controls. Nuclei are stained blue actin filaments red, PS green, PET orange. ( E ) Schematic diagram of the nanoplastic (NPLs) long-term exposure approach and analysis of their impact. ( F ) Quantification of the sphere-forming ability of untreated (CT) MCF10A cells or following 20 weeks of exposure to PS or PET nanoplastics (NPLs), n=5. ( G ) Quantification of total stem cell-derived progenitors by E-CFC assay , data represent the total number of colonies per 250 seeded cells, n=5. ( F , G , ) Each individual datum is represented. One way ANOVA is indicated on the graph by the P values. ( H ) Representation of the mean proportion of myoepithelial, mixed and luminal colonies quantified by E-CFC assay (n = 5). Data are expressed as the percentage of each subtype with a total number of scored colonies representing 100%. ( I ) Confocal images of acini. Nuclei are stained in blue, actin filaments in red, white arrows point to acini with a disorganized structure. Measurement of acini ( J ) solidity or ( K ) Ferret diameter ratio was quantified by image analysis using Fiji imaging software, n=109 to 131 individual acini. ( J , K ) Each individual data is represented. Mann-Whitney test is indicated on the graph by the P values.
Serumfree Medium Esf Sfm, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/serumfree medium esf sfm/product/Expression Systems Inc
Average 94 stars, based on 1 article reviews
serumfree medium esf sfm - by Bioz Stars, 2026-02
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Image Search Results


Representative confocal images of fluorescent PS nanoplastic internalization after the indicated time in: ( A ) primary bone marrow CD34+ sorted hematopoietic immature cells or ( B ) epithelial or fibroblast cells isolated from primary mammary gland obtained from healthy donors or ( C ) MCF10A-derived models representing non-transformed mammary stem cells (MCF10A-CT), early (MC26) and more transformed (M1B26) cells as previously described . Nuclei are stained in blue, actin filaments in red, PS in green. ( D ) Confocal images of PS and PET nanoplastic internalization in MCF10A after 24 h compared to non-exposed controls. Nuclei are stained blue actin filaments red, PS green, PET orange. ( E ) Schematic diagram of the nanoplastic (NPLs) long-term exposure approach and analysis of their impact. ( F ) Quantification of the sphere-forming ability of untreated (CT) MCF10A cells or following 20 weeks of exposure to PS or PET nanoplastics (NPLs), n=5. ( G ) Quantification of total stem cell-derived progenitors by E-CFC assay , data represent the total number of colonies per 250 seeded cells, n=5. ( F , G , ) Each individual datum is represented. One way ANOVA is indicated on the graph by the P values. ( H ) Representation of the mean proportion of myoepithelial, mixed and luminal colonies quantified by E-CFC assay (n = 5). Data are expressed as the percentage of each subtype with a total number of scored colonies representing 100%. ( I ) Confocal images of acini. Nuclei are stained in blue, actin filaments in red, white arrows point to acini with a disorganized structure. Measurement of acini ( J ) solidity or ( K ) Ferret diameter ratio was quantified by image analysis using Fiji imaging software, n=109 to 131 individual acini. ( J , K ) Each individual data is represented. Mann-Whitney test is indicated on the graph by the P values.

Journal: bioRxiv

Article Title: Chronic Exposure to Nanoplastics Alters Stem Cell Type-Specific Mechanisms, Promoting Cancer Development

doi: 10.64898/2026.01.21.700811

Figure Lengend Snippet: Representative confocal images of fluorescent PS nanoplastic internalization after the indicated time in: ( A ) primary bone marrow CD34+ sorted hematopoietic immature cells or ( B ) epithelial or fibroblast cells isolated from primary mammary gland obtained from healthy donors or ( C ) MCF10A-derived models representing non-transformed mammary stem cells (MCF10A-CT), early (MC26) and more transformed (M1B26) cells as previously described . Nuclei are stained in blue, actin filaments in red, PS in green. ( D ) Confocal images of PS and PET nanoplastic internalization in MCF10A after 24 h compared to non-exposed controls. Nuclei are stained blue actin filaments red, PS green, PET orange. ( E ) Schematic diagram of the nanoplastic (NPLs) long-term exposure approach and analysis of their impact. ( F ) Quantification of the sphere-forming ability of untreated (CT) MCF10A cells or following 20 weeks of exposure to PS or PET nanoplastics (NPLs), n=5. ( G ) Quantification of total stem cell-derived progenitors by E-CFC assay , data represent the total number of colonies per 250 seeded cells, n=5. ( F , G , ) Each individual datum is represented. One way ANOVA is indicated on the graph by the P values. ( H ) Representation of the mean proportion of myoepithelial, mixed and luminal colonies quantified by E-CFC assay (n = 5). Data are expressed as the percentage of each subtype with a total number of scored colonies representing 100%. ( I ) Confocal images of acini. Nuclei are stained in blue, actin filaments in red, white arrows point to acini with a disorganized structure. Measurement of acini ( J ) solidity or ( K ) Ferret diameter ratio was quantified by image analysis using Fiji imaging software, n=109 to 131 individual acini. ( J , K ) Each individual data is represented. Mann-Whitney test is indicated on the graph by the P values.

Article Snippet: Cells were seeded at a density of 1500 cells/mL on 96-well ultra-low-attachment plates (Corning) in serum free basal mammary epithelial medium (Promocell) supplemented with B27, 20 ng/mL epithelial growth factor (EGF), 20 ng/mL basic fibroblast growth factor (bFGF), and 4 μg/mL heparin.

Techniques: Isolation, Derivative Assay, Transformation Assay, Staining, Imaging, Software, MANN-WHITNEY